Fig. 4

PLS-DA and loading plot of mice model NMR results. a PLS-DA statistical analysis of the information provided by the Chenomx screening produced clear groupings, separating the controls (green), C. parvum Iowa II infections (blue) and C. parvum Weru infections (purple), 10 days post infection. As the grouping areas, indicated by the areas highlighted, do overlap, it can be said that the separation between the infection conditions represent some differences in the metabolome, which correspond to the C. parvum strain. b The loading biplot of the PLS-DA analysis shows many of the compounds identified by Chenomx contributed towards the separation and groupings. Those on the outer most edges, for example alanine, sarcosine, lactate and lactulose, had some of the greatest influence on the amount of separation as determined by the PLS-DA