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Fig. 2 | Gut Pathogens

Fig. 2

From: Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni

Fig. 2

Standard curve of recombinant HtrA and quantification of secreted HtrA in the supernatant of the C. jejuni 11168 and 81-176 cultures. a, b Standard curve for 11168 (a) and 81-176 (b) of one exemplary experiment based on the band intensities in Western blots against the concentrations of recombinant HtrA. The curve was plotted for each separate experiment based on the corresponding Western blot. c, d Overview of the secreted HtrA concentrations for 11168 (c) and 81-176 (d) in µg/mL and as pure numbers for the time points 2, 4 and 8 h. Values were calculated based on the standard curve for each blot separately and the error calculation was done based on the mean of these values (n = 5, error bars = SEM)

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