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Fig. 5 | Gut Pathogens

Fig. 5

From: Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni

Fig. 5

Western blot analysis of HtrA in the C. jejuni 11168 and 81-176 pellets and supernatants in the presence of Caco-2 cells. a Standard of recombinant HtrA in different dilutions from 10 to 0.25 µg/mL was blotted together with the C. jejuni supernatant samples and subjected to immunoblotting with antibodies against C. jejuni HtrA. Representative supernatant samples are shown of a C. jejuni culture taken at 0, 2, 4 and 8 h (right). b Corresponding C. jejuni pellet samples were also stained with α-HtrA antibodies. c Pellet and supernatant samples probed with α-FlaA antibodies as a negative control. d Pellet and supernatant samples stained with α-CiaB antibodies as a positive control for secreted proteins. We noted that CiaB in the extracellular fraction migrates at a slightly higher molecular weight (arrow), probably caused by a yet unknown posttranslational modification

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