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Table 2 Quantification of secreted HtrA in a time course (strain 81-176)

From: Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni

Time (h)

HtrA concentration (µg/mL)

Molecules of HtrA per mL (× 108)

Bacteria (× 109 CFU/mL)

Molecules HtrA secreted per CFU

Molecules HtrA secreted per CFU (with Caco-2 cells)

t = 2

0.5009 ± 0.1699

59,140 ± 20,070

1.7 ± 0.08

5483 ± 1,246

9640 ± 1078

t = 4

0.6604 ± 0.1623

77,980 ± 19,170

3.0 ± 0.26

3466 ± 814

5279 ± 1655

t = 8

0.8654 ± 0.2006

102,200 ± 23,690

5.1 ± 0.48

2151 ± 562

3263 ± 317

  1. Values were calculated for each experiment separately and then the mean of these values was calculated. The shown error values for the first three columns are SEM and based on the final mean values. The errors for the fourth and fifth column were calculated as described in the “ Materials and methods” section
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Gut Pathogens

ISSN: 1757-4749