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Fig. 3 | Gut Pathogens

Fig. 3

From: Cytokines derived from innate lymphoid cells assist Helicobacter hepaticus to aggravate hepatocellular tumorigenesis in viral transgenic mice

Fig. 3

ILC-derived cytokines contribute to helicobacter hepaticus-associated HCC development in HBs-Tg mice. Mice (4–5 month-old) were gavaged with H. hepaticus strain for 8 months. At 13 months old, hepatic cytokines were tested by qRT-PCR, hepatic ILCs in mice were analyzed by flow cytometry. a Relative mRNA expression of IFN-γ, IFN-γR1, IFN-γR2, IL-17, IL-22 and IL-23 (n = 6 in each groups). b Numbers of hepatic NK cell (CD3NK1.1+), NKT cell (CD3+NK1.1+) and T cell (CD3+NK1.1). c Numbers of hepatic Th17 (CD3+NK1.1CD4+IL-17+). Hepatic NK and NKT cells were gated to analyze the expression of phenotypical and functional molecules. d The expression of IFN-γ and IL-17 in intrahepatic NKT cells was analyzed by FACS. e The expression pattern of intrahepatic NK cells was analyzed by FACS. Data are expressed as median ± percentile. Mann–Whitney U was used. P < 0.05 represents statistical difference. *P < 0.05, **P < 0.01

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