Table 1 Primers used for amplification of the test genes and their sequences
Test gene | Primers used and their sequences | Expected PCR product size (bp) | Refs. | |
|---|---|---|---|---|
Cytotoxic necrotizing factor-1 | cnf-1 forward | 5′-GGGGGAAGTACAGAAGAATTA-3′ | 1112 | [2] |
cnf-1 reverse | 5′-TTGCCGTCCACTCTCACCAGT-3′ | |||
Colibactin A (phosphopantetheinyl transferase) | clbA forward | 5′-CTAGATTATCCGTGGCGATTC-3′ | 1002 | |
clbA reverse | 5′-CAGATACACAGATACCATTCA -3′ | |||
Colibactin Q (thioesterase enzyme) | clbQ forward | 5′-CTTGTATAGTTACACAACTATTTC-3′ | 821 | |
clbQ reverse | 5′-TTATCCTGTTAGCTTTCGTTC-3′ | |||