Skip to main content

Table 1 Primers used for amplification of the test genes and their sequences

From: Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli: experimental and bioinformatics analyses

Test gene Primers used and their sequences Expected PCR product size (bp) Refs.
Cytotoxic necrotizing factor-1 cnf-1 forward 5′-GGGGGAAGTACAGAAGAATTA-3′ 1112 [2]
cnf-1 reverse 5′-TTGCCGTCCACTCTCACCAGT-3′
Colibactin A (phosphopantetheinyl transferase) clbA forward 5′-CTAGATTATCCGTGGCGATTC-3′ 1002
clbA reverse 5′-CAGATACACAGATACCATTCA -3′
Colibactin Q (thioesterase enzyme) clbQ forward 5′-CTTGTATAGTTACACAACTATTTC-3′ 821
clbQ reverse 5′-TTATCCTGTTAGCTTTCGTTC-3′