Table 2 The phenotypic profile versus the genotypic profile of the test hemolytic isolates as compared to the reference E. coli strains 28C and J96
Isolate code | Phenotypic profile of test hemolytic E. coli isolates on cultured mammalian cells | Genotypic profile of test hemolytic E. coli isolates | ||||
|---|---|---|---|---|---|---|
Intact bacterial cells (megalocytosis) | Crude cell lysate (multinucleation/cytotoxicity) | |||||
Caco2 cells | Hela cells | Vero cells | cnf 1 | clb A | clb Q | |
1β | − | − | − | − | − | − |
3β | − | − | − | − | − | − |
4β | + | +* | +* | + | + | + |
5β | − | − | − | + | − | − |
6β | − | − | − | + | + | + |
10β | − | +* | +* | + | + | + |
12β | − | − | − | − | − | − |
13β | − | + | + | − | − | − |
19β | − | − | − | − | − | − |
27β | + | +* | +* | + | + | + |
28β | + | + * | − | + | + | + |
29β | + | +* | − | + | + | + |
32β | − | + | + | – | – | – |
55β | − | + | + | – | – | – |
70β | + | + * | +* | + | + | + |
85β | − | − | − | − | − | − |
88β | − | − | − | − | − | − |
99β | + | + | + | − | + | + |
100β | − | + | + | − | − | − |
108β | − | + | + | − | − | − |
114β | + | +* | +* | + | + | + |
E. coli strain J96 | + | +* | +* | + | + | + |
E. coli strain 28C | + | +* | +* | + | + | + |
Percent out of the hemolytic isolates | 7 (33.33%) | 13 (61.9%) | 10 (47.6%) | |||
cnf-1+ clb A+ clb Q+ clb M+ hly+ | 8/21 (38.1%) | |||||
clb A + clb Q + clb M + hly + only | 1/21 (4.7%) | |||||
cnf-1+ hly+ only | 1/21 (4.7%) | |||||