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Table 2 The phenotypic profile versus the genotypic profile of the test hemolytic isolates as compared to the reference E. coli strains 28C and J96

From: Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli: experimental and bioinformatics analyses

Isolate code Phenotypic profile of test hemolytic E. coli isolates on cultured mammalian cells Genotypic profile of test hemolytic E. coli isolates
Intact bacterial cells (megalocytosis) Crude cell lysate (multinucleation/cytotoxicity)
Caco2 cells Hela cells Vero cells cnf 1 clb A clb Q
+ +* +* + + +
+
+ + +
10β +* +* + + +
12β
13β + +
19β
27β + +* +* + + +
28β + + * + + +
29β + +* + + +
32β + +
55β + +
70β + + * +* + + +
85β
88β
99β + + + + +
100β + +
108β + +
114β + +* +* + + +
E. coli strain J96 + +* +* + + +
E. coli strain 28C + +* +* + + +
Percent out of the hemolytic isolates 7 (33.33%) 13 (61.9%) 10 (47.6%)
cnf-1+ clb A+ clb Q+ clb M+ hly+ 8/21 (38.1%)
clb A + clb Q + clb M + hly + only 1/21 (4.7%)
cnf-1+ hly+ only 1/21 (4.7%)
  1. +, presence of character (cytotoxicity or gene); −, absence of character (cytotoxicity or gene); +* indicates the appearance of multinuclei (multinucleation); + only in crude cell lysate cytotoxicity indicates general uncharacteristic cytotoxic effect