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Table 3 Pathologic effects exerted by the hemolytic test isolates on the kidneys and bladders of female rats

From: Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli: experimental and bioinformatics analyses

Pathological changes Test isolatesa
70β 13β 67
Kidneys
 Interstitial blood vessels
  Degree of congestion ++++ ++ + +
 Inflammatory cells infiltrations
  Degree of infiltration ++++ ++ + +
 Renal tubular epithelium
  Edema/swelling ++++ ++ + +
  Vacuolar degeneration ++++ ++ + +
  Necrosis ++++ ++ + +
  Nuclear pyknosis ++++ +
  Thickening of bowman’s capsule ++++
 Renal glomeruli
  Congestion of capillaries ++++ ++ +
  Edema of glomerular tuft ++++ ++ +
  Necrosis of glomerular tuft ++++ ++ +
  Hyalinization of the glomerular tuft ++++ ++ +
Bladder
 Lamina propria
  Edema ++++ ++
  Inflammatory cells infiltration ++++ ++ + +
 Transitional epithelium
  Hyperplastic changes ++++ ++
  Necrosis ++++ ++
  Desquamation ++++
  Vacuolar degeneration ++++ ++ + +
  1. +, mild; ++, moderate; ++++ , severe
  2. aCharacteristics of the test isolate: 70β; cnf-1+ clb A+ clb Q+; shows beta-hemolysis on blood agar; crude cell lysate and viable cell cytotoxicity in mammalian cell. 6β; cnf-1+ clb A+ clb Q+; shows beta-hemolysis on blood agar; no cytotoxicity in mammalian cells. 13β; cnf-1 clb A clb Q; shows beta-hemolysis on blood agar and mammalian cell cytotoxicity of crude cell lysate only. 67; cnf-1 clb A clb Q; shows neither hemolysis on blood agar nor cytotoxicity in mammalian cells