Extensive inter-strain diversity among clinical isolates of Shigella flexneri with reference to its serotype, virulence traits and plasmid incompatibility types, a study from south India over a 6-year period

Table 4 List of primers used in the study

Gene target	Primer sequence (5′-3′)	Gene location	Amplicon size in base pair (bp)	Reference
ipaH	F:TGGAAAAACTCAGTGCCTCT	Plasmid and chromosome	423	[40]
ipaH	R:CCAGTCCGTAAATTCATTCT	Plasmid and chromosome	423	[40]
ial	F:CTGGATGGTATGGTGAGG	Plasmid	320	[40]
ial	R:GGAGGCCAACAATTATTTCC	Plasmid	320	[40]
set1A	F:TCACGCTACCATCAAAGA	SHI-1 pathogenicity island	309	[40]
set1A	R:TATCCCCCTTTGGTGGTA	SHI-1 pathogenicity island	309	[40]
set1B	F:GTGAACCTGCTGCCGATATC	SHI-1 pathogenicity island	147	[40]
set1B	R:ATTTGTGGATAAAAATGACG	SHI-1 pathogenicity island	147	[40]
sen	F:ATGTGCCTGCTATTATTTAT	Plasmid	799	[40]
sen	R:CATAATAATAAGCGGTCGC	Plasmid	799	[40]
virF	F:CAATGACGGTTAGCTCAGGCA	Plasmid	450	This study
virF	R: AAAGACGCCATCTCTTCTCGAT	Plasmid	450	This study
ompA	F:GCAGGCATTGCTGGGTAA	Plasmid	1319	[36]
ompA	R:ACACTTGTAAGTTTTCAACTACG	Plasmid	1319	[36]

ISSN: 1757-4749