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Fig. 3 | Gut Pathogens

Fig. 3

From: Validating the inhibitory effects of d- and l-serine on the enzyme activity of d-3-phosphoglycerate dehydrogenases that are purified from Pseudomonas aeruginosa, Escherichia coli and human colon

Fig. 3

Optimum pH of the purified PGDHs derived from three different species. The optimal activity (mU/mg) of each PGDH was determined by performing the PGDH activity assay in the reaction mixture containing 100 mM Tris–HCl buffer at various pH. The assay was performed in triplicate, and data being shown as the mean activity (mU/mg) ± SD. a A significant difference in the activity of P. aeruginosa PGDH was observed between at pH 7.5 versus pH 8.5 and pH 9.0 (*P < 0.05), but not at pH 7.5 versus pH 8.0 (ns: not significant, P > 0.05). b A significant difference was observed in the activity of E. coli PGDH measured at pH 8.5 versus pH 7.5 and pH 8.0, (*P < 0.05), but not at pH 8.5 versus pH 9.0 (ns). c A significant difference was observed in the activity of human PGDH measured at pH 9.0 versus pH 7.5 and pH 8.0 (*P < 0.05), but not at pH 8.5 versus pH 9.0 (ns)

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Gut Pathogens

ISSN: 1757-4749