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Fig. 3 | Gut Pathogens

Fig. 3

From: Nonhemolysis of epidemic El Tor biotype strains of Vibrio cholerae is related to multiple functional deficiencies of hemolysin A

Fig. 3

ST.4/ST.5 genes have no role in conferring hemolysis ability to the strains. CΔhlyA-CST.4, the strain complemented with pBAD33-ST.4 into CΔhlyA, remained non-hemolytic, whereas when VC1279 (ST.4 type of hlyA) and VC2568 (ST.5 type of hlyA) were complemented with pBAD33-hlyA to generate strain VC1279(pBAD33-hlyA) and VC2568(pBAD33-hlyA) respectively, their hemolysis tests became positive. The positive control was strain C6706, and the negative control was strain CΔhlyA. The 1% sheep erythrocyte LB solution was used as the blank control

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