Table 3 Strains and plasmids used in this study
Strains/plasmids | Characters | Reference |
|---|---|---|
V. cholerae | ||
 C6706 | El Tor biotype, serogroup O1, Smr | Lab collections |
 CΔhlyA | hlyA deletion of C6706, Smr | This study |
 CΔhlyA-C | CΔhlyA strains was complemented with pBAD33-hlyA | This study |
 CΔhlyA-CST.4 | CΔhlyA strains was complemented with pBAD33-ST.4 | This study |
 CΔhlyA-CST.5 | CΔhlyA strains was complemented with pBAD33-ST.5 | This study |
 CΔhlyA-CK | CΔhlyA strains was complemented with pBAD33 for control | This study |
E. coli | ||
 SM10λpir | Km, thi thr leu tonA lacY supE recA::RP4-2-TC::Mu λpir | Lab collections |
 DH5αλpir | supE44 ΔlacU169 (ΦlacZΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1 λpir | Lab collections |
Plasmids | ||
 pWM91 | Suicide plasmid; oriR oriT lacZ tetAR sacB, AMPr | Lab collections |
 pWM91-ΔhlyA | pWM91 carrying upstream and downstream fragments flanking hlyA | This study |
 pBAD33 | E. coli expression vector, Cmr | Lab collections |
 pBAD33-hlyA | pBAD33 containing hlyA, Cmr | This study |
 pBAD33-ST.4 | pBAD33 containing ST.4 hlyA, Cmr | This study |
 pBAD33-ST.5 | pBAD33 containing ST.5 hlyA, Cmr | This study |