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Fig. 1 | Gut Pathogens

Fig. 1

From: Recombinant fusion protein of Heparin-Binding Hemagglutinin Adhesin and Fibronectin Attachment Protein (rHBHA-FAP) of Mycobacterium avium subsp. paratuberculosis elicits a strong gamma interferon response in peripheral blood mononuclear cell culture

Fig. 1

SDS-PAGE (a) and western blot (b) of chimeric rHBHA-FAP. a Running samples on 12% SDS-PAGE gel for choosing optimum temperature of rHBHA-FAP expression in E. coli. Lane M: protein ladder (CinnaGen PR911654 [SL7012]), lane 1: negative control, lane 2: expression at 30 °C, lane 3: expression at 37 °C. The white arrow indicates our desired band. b Western blot analysis using HRP-anti-polyhistidine. Lanes 1, 2 and 3: rHBHA-FAP with the expected size of ~ 35 kDa, lane 4: negative control

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Gut Pathogens

ISSN: 1757-4749