Fig. 4

In silico analyses of cdtA, B and C gene structures in C. coli. a Schematic picture showing intact cdtA, B and C ORFs in the blood (B5, B49 and B63) and stool (F3, F4 and F8) isolates as well as the reference strains RM2228 and LMG 6440 as grey boxed arrows. Letters denote nucleotide insertions, while crossed-over letters denote deletions in the nucleotide sequences (black lines). b All identified mutations leading to disrupted ORFs in the cdt genes of the 128 clade 1 sequences that were used in the phylogenetic analysis. Numbers above the ORFs indicate the position of each mutation, letters denote nucleotide insertions, crossed-over letters denote nucleotide deletions and the prevalence of each specific mutation is indicated as a percentage. Percentage of the isolates in each group (blood, n = 34; stool, n = 38; and animal, n = 54) with all (c) and each (d) cdt ORF intact, respectively