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Table 3 The Vibrant GPP array’s lowest limit of detection

From: Evaluation of the Vibrant DNA microarray for the high-throughput multiplex detection of enteric pathogens in clinical samples

  Organism Source/isolate ID LoD concentration Agreement
Bacteria E. coli O157:H7 ATCC 43895 1.0 × 102 cells/mL 100% (5/5)
Enteroaggregative E. coli (EAEC) ATCC 23501 1.0 × 102 cells/mL 100% (5/5)
Enteropathogenic E. coli (EPEC) ATCC 43887, Clinical Specimen 1.5 × 103 cells/mL 100% (5/5)
Enterotoxigenic E. coli (ETEC) lt/st ATCC 35401 2.0 × 103 cells/mL 100% (5/5)
Shiga-like toxin producing E. coli (STEC) stx1/stx2 ATCC BAA-2196, ATCC 43895, Clinical Specimen 1.0 × 102 cells/mL 100% (5/5)
Plesiomonas shigelloides ATCC 14029 3.0 × 102 cells/mL 100% (5/5)
Vibrio parahaemolyticus ATCC 17802 3.0 × 103 cells/mL 100% (5/5)
Vibrio vulnificus ATCC 27562 1.0 × 104 cells/mL 100% (5/5)
Helicobacter pylori ATCC 700392 1.5 × 104 cells/mL 100% (5/5)
Listeria spp. ATCC 19111 3.0 × 103 cells/mL 100% (5/5)
Vibrio Cholerae ATCC 14035 2.0 × 102 cells/mL 100% (5/5)
C. difficile Toxin A/B ATCC 9689 1.0 × 103 cells/mL 100% (5/5)
Salmonella spp. ATCC 700623 2.0 × 103 cells/mL 100% (5/5)
Shigella/Enteroinvasive E. coli (EIEC) ATCC 29930 1.0 × 102 cells/mL 100% (5/5)
Yersinia enterocolitica ATCC 9610 2.0 × 104 cells/mL 100% (5/5)
Campylobacter jejuni ATCC BAA-1234 3.0 × 102 cells/mL 100% (5/5)
Campylobacter upsaliensis ATCC 43954 1.0 × 102 cells/mL 100% (5/5)
Parasite Giardia lamblia ATCC 30957 4.0 × 102 cells/mL 100% (5/5)
Cryptosporodium spp. Waterborne P102C 1.0 × 102 oocycts/mL 100% (5/5)
Entamoeba histolytica ATCC 30459 1.0 × 102 cells/mL 100% (5/5)
Cyclospora cayetanensis Zeptometrix control 2.0 × 103 DNA copies/mL 100% (5/5)
Virus Norovirus GI/GII Zeptometrix control 1.0 × 103 RNA copies/mL 100% (5/5)
Rotavirus A ATCC VR-2104 3.1 × 102 RNA copies/mL 100% (5/5)
Adenovirus F 40/41 ATTC VR-930/931 1.0 × 102 RNA copies/mL 100% (5/5)
Astrovirus Zeptometrix control 1.2 × 103 RNA copies/mL 100% (5/5)
Sapovirus (I, II, IV, V) Zeptometrix control 2.1 × 102 RNA copies/mL 100% (5/5)
Fungi Candida spp. ATCC 10231 1.0 × 102 cells/mL 100% (5/5)
Microsporidium spp. Clinical specimen 2.0 × 102 DNA copies/mL 100% (5/5)