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Fig. 6 | Gut Pathogens

Fig. 6

From: RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagic Escherichia coli O157:H7

Fig. 6

EMSA of the binding of RstA. EMSA of the binding of RstA to PLEE1 (a), PLEE2/3 (b), PLEE4 (c), and PLEE5 (d), rpoS (e, negative control), Pasr (f), PhdeA (g) and PyeaI (h). PCR products were added to the reaction mixtures at 40 ng each. RstA protein was added to the reaction buffer in each assay in lanes 2–5 at 0.25, 0.5, 1, and 2 μM, respectively. No protein was added in lane 1. No binding was observed in ae, while binding was observed in fh

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