Fig. 2
From: Hypervirulent clade 2, ribotype 019/sequence type 67 Clostridioides difficile strain from Japan
Cytotoxicity of Clostridioides difficile strain B18-123 on Vero cells. (a) Vero cell viability in B18-123 was evaluated together with positive control ATCC BAA-1870 (NAP1/RT027/ST1; TcdA+, TcdB+, Cdt+) and negative control ATCC 700057 (RT038/ST48; TcdA−, TcdB−, Cdt−) strains, as described previously [16]. The results are presented as means ± standard deviations of cell viability percentages. (b) Cytotoxic effect of C. difficile strains studied under a light microscope (original magnification × 200)