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Table 1 Primer used for the detection of tet(O) and tet(A)

From: Investigation of molecular mechanisms underlying tetracycline resistance in thermophilic Campylobacter spp. suggests that previous reports of tet(A)-mediated resistance in these bacteria are premature

Primer

Sequence (5′–3′)

Amplicon size (bp)

Annealing temperature (°C)

References

TetO-FW

ACGGARAGTTTATTGTATACC

171

52

[6]

TetO-RV

TGGCGTATCTATAATGTTGAC

Tet(A)-F

GTGAAACCCAACATACCCC

888

50

[1]

Tet(A)-R

GAAGGCAAGCAGGATGTAG

[7]

Tet(A)-Camp-F

ATCGTGGCCGGCATCACCGG

407

54

This study

Tet(A)-Camp-R

TCCTCGCCGAAAATGACCC