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Fig. 2 | Gut Pathogens

Fig. 2

From: Characterization of Arcobacter strains isolated from human stool samples: results from the prospective German prevalence study Arcopath

Fig. 2

Viability of HT-29/B6 cells after inoculation with Arcobacter isolates. HT-29/B6 cells, differentiated for 7 days, were inoculated with A. butzleri (a), A. cryaerophilus (b) and A. lanthieri (c) isolates (MOI 100, except for isolates 02651 and 02771 that were incubated at MOI 50), and cytotoxicity was measured after 48 h incubation by WST-1 assay. At least three independent experiments were performed with six replicates each. Cells treated with medium only or with A. cryaerophilus ILSH 02659 (Ac) were included as negative control, and dimethyl sulfoxide (DMSO) and Campylobacter jejuni 81–176 (Cj) as positive controls. The isolates were arbitrarily classified in three groups due to the level of toxicity, i.e., isolates of low (group I), moderate (group II) and high cytotoxicity (group III) with 20–49%, 50–94% and at least 95% reduction of absorbance as compared to the negative control, respectively. # inoculation at MOI 50; * p < 0.05 (Mann–Whitney U-test) as compared to control

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