Fig. 1

Genomic locus, transposon insertion sites in C. jejuni NCTC 11168 mutants ::Cj1492c and ::Cj1507c and PCR verification of both mutants and phenotypic analyses of two mutants. a, b Genes are plotted as arrows in order to their genomic locations. Boxed arrows length indicates relative gene sizes. a The location of inserted transposon is indicated in C. jejuni two-component sensor gene (Cj1492c). b The location of the inserted transposon is indicated in C. jejuni regulatory protein gene (Cj1507c). c Agarose gel electrophoresis of genomic DNA of mutants ::Cj1492c, ::Cj1507c and wild type using gene specific primers. With integration of the transposon (1219 base pairs) the DNA fragment sizes of both mutants are around 1400 base pairs in length which are 1.2 kb larger than the target DNA of the wild type (around 200 base pairs). d Growth curve of wild type, ::Cj1492c and ::Cj1507c were assessed by CFU counting. Values are shown as mean value of three independent experiments. Error bars represent SD. Statistical comparison with the wild type: *p ≤ 0.05, unpaired t-test. e Differential swarming ability of mutated strains was normalised with the wild type strain and is presented as percentage of swarming halos of wild type (100%). Columns show median value of five biological replicates and bars indicate maximum and minimum range. *p ≤ 0.05; ***p ≤ 0.001; ****p ≤ 0.0001, unpaired t-test