Fig. 2
From: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
Adhesion, invasion and internalisation levels of C. jejuni in HT-29/B6 cells and immunofluorescent detection of intracellular C. jejuni. Adhesion (a) of three tested strains to HT-29/B6 was detected at 1 h p.i.. For determination of invasion (b), the monolayer of HT-29/B6 was incubated with gentamicin for an additional 2 h prior to lysis. Experiments were performed at least three independent times in triplicate. The relative adhesion and invasion index were normalised with wild type and are presented as percentage of the adhesion and invasion index of wild type, respectively. Results show the mean value ± SD. *p ≤ 0.05; ***p ≤ 0.001, unpaired t-test. c HT-29/B6 cells and immunofluorescently labelled wild type, ::Cj1492c and ::Cj1507c (green, indicated by white arrows) and untreated control cells are shown at 5 h p.i. Cell cytoplasm membrane were stained with WGA-CF®594 (red) and nuclei with DAPI (blue). Scale bars indicates 25 μm. d The internalisation level of C. jejuni by HT-29/B6 was determined as a ratio of particles of invaded C. jejuni and infected cells by means of immunofluorescence microscopy and automatic particle counting using ImageJ. The relative level of internalisation was normalised with wild type and is presented as percentage of the wild type. Fluorescent particles of each strain were quantified in at least six microphotographs selected randomly for each biological replication and results are presented for four individual experiments. Asterisks show statistically significant differences between ::Cj1492c and ::Cj1507c. *p ≤ 0.05; unpaired t-test