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Fig. 3 | Gut Pathogens

Fig. 3

From: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion

Fig. 3

Intracellular survival of C. jejuni, relative viability of HT-29/B6 cells and RT-qPCR analysis of IL-6 and IL-8 mRNA expression in HT-29/B6 cells after C. jejuni infection. a Intracellular survival, presented as CFU recovery rates of intracellular viable cell counts, was determined as a ratio of viable counts between 5 and 24 h p.i. The relative survival rate was normalised with the wild type and is presented as percentage of the wild type. Results represent at least six individual experiments and asterisks indicate statistically significant differences between mutants and the wild type. *p ≤ 0.05; **p ≤ 0.01, unpaired t-test. b Relative viability of HT-29/B6 cells after infection was measured after 5 h and 24 h of infection and calculated as ratio of number of viable cells compared to the total cell numbers and normalised on the viability of uninfected control group. Values are the mean ± SD of three individual experiments. c Fold changes of IL-6 and IL-8 mRNA expression were calculated relatively to non-infected controls and normalized to housekeeper. Experiments were performed in triplicate and the mean ± SD is presented. Only altered mRNA expression level above or below the dotted lines are defined as regulated. Asterisks indicate statistically significant differences between tested strains and negative controls at each time point. *p ≤ 0.05; **p ≤ 0.01; ***p≤ 0.001, unpaired t-test

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