Fig. 5
The effects of sodium fumarate on C. concisus growth under anaerobic conditions with and without H2 gas. C. concisus strains P2CDO4, H17O-S1, and P26UCO-S2 were cultured on two sets of HBA, HBAfum0.05, HBAfum0.2 and HBAfum0.4. One set was incubated in anaerobic conditions with hydrogen and the other was incubated without hydrogen. Both the starting number of bacteria and total after incubation was enumerated for all strains. Fold changes were calculated relative to the starting number of bacteria from quadruplicate counts. In anaerobic conditions with hydrogen, all strains showed increased growth, with the greatest increase on HBAfum0.4. In anaerobic conditions without hydrogen, all strains had a CFU lower than the initial inoculation CFU. *Indicates statistical significance compared to CFU on HBA in the same condition. *** P < 0.001