Fig. 4

Keap1 silencing reduces ROS generation, apoptosis, and inflammation in EV71-infected cells. Vero and RD cells were infected by EV71 at an MOI of 5 subsequent to 24 h transfection using Keap1 siRNA vector. a DCF fluorescence intensity indicated ROS generation in the infected RD and Vero cells in comparison to non-infected normal mice (Control). b, c Quantity of dead RD as well as Vero cells using Annexin V-FITC and PI flow cytometry. The upper right quadrant of every plot stood for early dead cells. d–f Expression of inflammation-promoting cytokines IL-1β, IL-6, and TNF-α in the infected cells were assessed by qPCR. g–i The protein expression levels of IL-1β, IL-6, and TNF-α of the infected cells were quantified using ELISA. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Indicated group