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Fig. 5 | Gut Pathogens

Fig. 5

From: EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cells

Fig. 5

Keap1 and Nrf2 co-silencing blocks Keap1–Nrf2–HO-1 transduction in EV71-infected cells. Subsequent to 24 h co-transfection with Keap1 and Nrf2 siRNA vectors, Vero and RD cells were infected by EV71 at an MOI of 5. a, b Western blot was used to examine the expression of Keap1, Nrf2, and HO-1 proteins in infected and non-infected cells. ce qPCR was used to assess the mRNA of Keap1, Nrf2, and HO-1 in infected and non-infected cells. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs. Indicated group

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