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Fig. 6 | Gut Pathogens

Fig. 6

From: EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cells

Fig. 6

Nrf2 silencing restores ROS generation, apoptosis, and inflammation of EV71-infected cells with Keap1 silencing. Subsequent to 24 h co-transfection with the Keap1 and Nrf2 siRNA vector, Vero and RD cells were infected by EV71 at an MOI of 5. a DCF fluorescence intensity indicated ROS generation in infected RD and Vero cells in comparison to the non-infected normal mice (Control). b, c Dead RD and Vero cells were enumerated using Annexin V-FITC and PI flow cytometry. The upper right quadrant of every plot stood for early dead cells. df Expression of inflammation-promoting cytokines IL-1β, IL-6, and TNFα in the infected cells were assessed using qPCR. gi The protein expression levels of IL-1β, IL-6, and TNF-α of the infected cell were quantified using ELISA. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Indicated group

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