Fig. 2
From: Host responses to Clostridium perfringens challenge in a chicken model of chronic stress
Clostridium perfringens infection modulated mucus production. Birds were administered 0.2% ethanol drinking water (Cp−St−), challenged with 107 CFU C. perfringens (Cp+St−), administered 20 mg/L CORT (Cp−St+), or received both C. perfringens and CORT challenge (Cp+St+). Treatments commenced in birds at 14-days-of-age, where C. perfringens was administered for 2 days and CORT for 7 days. a Example of a duodenal tissue section stained with alcian blue periodic acid Schiff stain; an individual villus and crypt are indicated with the dotted lines. b, c ImageJ quantification of alcian blue staining presented as percent surface area of alcian blue staining to total area of villi or crypt region. b Villi alcian blue staining in the duodenum and jejunum. c Crypt alcian blue staining in the duodenum and jejunum. d Relative mRNA gene quantity of MUC2B and MUC5AC in the duodenum and jejunum. Vertical lines associated with histogram bars represent standard error of the means (n = 4). Asterisks indicate significant differences (P < 0.050)