Fig. 2

CagA internalization in host cells upon H. pylori infection visualized by Western blots. a–d Show Western blots stained with α-PY-99 antibodies for detection of internalized, phosphorylated CagA^PY on top (CagA^PY position indicated by an arrow), non-phosphorylated CagA detected by α-CagA antibodies as a bacterial loading control in the middle, and the β-actin protein loading control at the bottom, followed by the relative fraction of CagA^PY as determined by densitometry. a Shows AGS cells infected with the seven wild-type H. pylori strains plus the ∆cagY mutant for 6 h, and b–d) show those results for HN, CAL-27 and BHY cells. The asterisks in the α-PY-99-stained blots indicate the position of a ~ 125 kDa host cell protein