Fig. 3From: Type IV secretion of Helicobacter pylori CagA into oral epithelial cells is prevented by the absence of CEACAM receptor expressionIn vitro phosphorylation assay of CagA after infection of oral cell lines. a HN, CAL-27, and BHY cells were infected with indicated H. pylori strain for 6 h, followed by harvesting and in vitro phosphorylation assay in the presence or absence of NP-40 as described in the Experimental procedures. CagA phosphorylation was verified using Western blots. The asterisk indicates the position of a phosphorylated ~ 125 kDa host cell protein. b CagAPY signals were quantified by densitometric measuring of band intensitiesBack to article page