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Fig. 6 | Gut Pathogens

Fig. 6

From: Type IV secretion of Helicobacter pylori CagA into oral epithelial cells is prevented by the absence of CEACAM receptor expression

Fig. 6

Transient expression of CEACAM1, 5 and 6 in oral epithelial cell lines restores CagA internalization. The oral cell lines HN (a), CAL-27 (b) and BHY (c) were transfected with CEACAM1, CEACAM5 or CEACAM6 or with an empty vector control, as indicated by the ± signs (top). The cells were then infected for 6 h with H. pylori strain HPAG1. Total cell lysates were subjected to Western blotting stained with specific monoclonal antibodies to identify expression of CEACAM1, 5 or 6, respectively (blots shown on top) and with α-PY99 and α-CagA to demonstrate CagA internalization in the oral cells as a result of CEACAM expression. df CagA internalization was quantified by band intensities from three independent experiments

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