Fig. 1

Melting curves a, melting peaks b, normalized melting peaks c, and differential curves d of reference plasmids of Blastocystis spp., Cryptosporidium spp., D. fragilis, E. histolytica, G. intestinalis assemblage B and G. intestinalis assemblage A. The following melting temperatures were observed: 71.5 °C for D. fragilis (brown), 75.5 °C for E. histolytica (green), 78.5 °C for Cryptosporidium spp. (lila), 80 °C for Blastocystis spp. (blue), 81.5 °C for G. intestinalis assemblage B (light red), and 83.5 °C for G. intestinalis assemblage A (red). The distance between the melting curves is well specified in the difference curve graph d