Table 1 List of primers for amplification of the long original and the short new fragments, with fragment sizes and target genes for each parasite species

Dientamoeba fragilis

Long fragment

886 bp

[9]

Dienta F: 5′-TAT CGG AGG TGG TAA TGA CC-3′

Dienta R: 5′-CAT CTT CCT CCT GCT TAG ACG-3′

SSU rRNA

Short fragment

114 bp

Our study

Dienta F2: 5′-CAA ATC AGA ACG CTT AAA GTA ATT TTC-3′

Dienta R2: 5′-CCC CGA TTA TTC TCT TTG ATA TT-3′

Entamoeba histolytica

Long fragment

167 bp

[20]

EntaF: 5′-ATG CAC GAG AGC GAA AGC AT-3′

EhR: 5′-GAT CTA GAA ACA ATG CTT CTC T-3′

SSU rRNA

Short fragment

96 bp

Our study

EntaF2: 5′-CGA TCA GAT ACC GTC GTA GTC C-3′

EhR: 5′-GAT CTA GAA ACA ATG CTT CTC T-3′

Cryptosporidium spp.

Long fragment

~161 bp

[21]

Crypto F: 5′-AGT GAC AAG AAA TAA CAA TAC AGG -3′

Crypto R: 5′-CCT GCT TTA AGC ACT CTA ATT TTC-3′

SSU rRNA

Short fragment

~88 bp

Our study

Crypto_Frag_F: 5′-GTG ACA TAT CAT TCA AGT TTC TGA CC-3′

Crypto_Frag_R: 5′-TAA TTC CCC GTT ACC CGT CA-3′

Confirmation qPCR-HRM

138 bp

[9]

CrF 5′-CGC TTC TCT AGC CTT TCA TGA-3

CrR 5′-CTT CAC GTG TGT TTG CCA AT-3

Blastocystis spp.

Long fragment

320-342 bp

[22]

Bl_18rRNA F: 5′-AGT AGT CAT ACG CTC GTC TCA AA-3′

Bl_18rRNA R: 5′-TCT TCG TTA CCC GTT ACT GC-3′

SSU rRNA

Short fragment

82 bp

Our study

Bl_18rRNA_F1: 5′-GCA GTA ACG GGT AAC GAA GAA-3′

Bl_18rRNA_R1: 5′-TGC TGC CTT CCT TGG ATG T-3′

Giardia intestinalis

Long fragment

~432 bp

[23]

GDHiF: 5′-CAG TAC AAC TCY GCT CTC GG-3

GDHiR: 5′-GTT RTC CTT GCA CAT CTC C-3′

gdh

Short fragment

~133 bp

Our study

Giar-GDH-F3: 5′-GGC AAG AAC RTC AAG TGG-3′

Giar-GDH-R2: 5′-TTG TCC TTG CAC ATC TCC TC-3′