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Table 1 List of primers for amplification of the long original and the short new fragments, with fragment sizes and target genes for each parasite species

From: A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites

Species & GenePrimersPCR productReference
Dientamoeba fragilisLong fragment886 bp[9]
Dienta F: 5′-TAT CGG AGG TGG TAA TGA CC-3′  
Dienta R: 5′-CAT CTT CCT CCT GCT TAG ACG-3′  
SSU rRNAShort fragment114 bpOur study
Dienta F2: 5′-CAA ATC AGA ACG CTT AAA GTA ATT TTC-3′  
Dienta R2: 5′-CCC CGA TTA TTC TCT TTG ATA TT-3′  
Entamoeba histolyticaLong fragment167 bp[20]
EntaF: 5′-ATG CAC GAG AGC GAA AGC AT-3′  
EhR: 5′-GAT CTA GAA ACA ATG CTT CTC T-3′  
SSU rRNAShort fragment96 bpOur study
EntaF2: 5′-CGA TCA GAT ACC GTC GTA GTC C-3′  
EhR: 5′-GAT CTA GAA ACA ATG CTT CTC T-3′  
Cryptosporidium spp.Long fragment~161 bp[21]
Crypto F: 5′-AGT GAC AAG AAA TAA CAA TAC AGG -3′  
Crypto R: 5′-CCT GCT TTA AGC ACT CTA ATT TTC-3′  
SSU rRNAShort fragment~88 bpOur study
 Crypto_Frag_F: 5′-GTG ACA TAT CAT TCA AGT TTC TGA CC-3′  
Crypto_Frag_R: 5′-TAA TTC CCC GTT ACC CGT CA-3′  
Confirmation qPCR-HRM138 bp[9]
CrF 5′-CGC TTC TCT AGC CTT TCA TGA-3  
CrR 5′-CTT CAC GTG TGT TTG CCA AT-3  
Blastocystis spp.Long fragment320-342 bp[22]
Bl_18rRNA F: 5′-AGT AGT CAT ACG CTC GTC TCA AA-3′  
Bl_18rRNA R: 5′-TCT TCG TTA CCC GTT ACT GC-3′  
SSU rRNAShort fragment82 bpOur study
Bl_18rRNA_F1: 5′-GCA GTA ACG GGT AAC GAA GAA-3′  
Bl_18rRNA_R1: 5′-TGC TGC CTT CCT TGG ATG T-3′  
Giardia intestinalisLong fragment~432 bp[23]
GDHiF: 5′-CAG TAC AAC TCY GCT CTC GG-3  
GDHiR: 5′-GTT RTC CTT GCA CAT CTC C-3′  
gdhShort fragment~133 bpOur study
Giar-GDH-F3: 5′-GGC AAG AAC RTC AAG TGG-3′  
Giar-GDH-R2: 5′-TTG TCC TTG CAC ATC TCC TC-3′