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Table 2 Multiplex melting temperatures (Tm), specificities and limits of detection (LOD)

From: A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites

SpeciesTm (Tm range) (°C)Efficiency (%)SlopeR2LOD (Min–Max) copy/µl
Dientamoeba fragilis71.50 ± 0.00 (71.5–7 1.5)98.97− 3.34680.997511.26 (7.03–25.15)
Entamoeba histolytica75.20 ± 0.25 (75.0–75.5)97.05− 3.39480.999830.08 (18.02–69.13)
Cryoptosporidium spp.78.23 ± 0.25 (78.0–78.5)103.11− 3.24970.99888.78 (6.06–15.99)
Blastocystis spp.79.84 ± 0.23 (79.5–80.0)97.60− 3.38080.997410.95 (7.08–22.66)
Giardia intestinalis assemblage B81.51 ± 0.08 (81.5–82.0)95.77− 3.42770.996920.92 (15.60–37.15)
Giardia intestinalis assemblage A83.50 ± 0.00 (83.5–83.5)96.76− 3.40220.994220.00 (15.24–33.62)