Table 2 Multiplex melting temperatures (Tm), specificities and limits of detection (LOD)
From: A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites
Species | Tm (Tm range) (°C) | Efficiency (%) | Slope | R2 | LOD (Min–Max) copy/µl |
|---|---|---|---|---|---|
Dientamoeba fragilis | 71.50 ± 0.00 (71.5–7 1.5) | 98.97 | − 3.3468 | 0.9975 | 11.26 (7.03–25.15) |
Entamoeba histolytica | 75.20 ± 0.25 (75.0–75.5) | 97.05 | − 3.3948 | 0.9998 | 30.08 (18.02–69.13) |
Cryoptosporidium spp. | 78.23 ± 0.25 (78.0–78.5) | 103.11 | − 3.2497 | 0.9988 | 8.78 (6.06–15.99) |
Blastocystis spp. | 79.84 ± 0.23 (79.5–80.0) | 97.60 | − 3.3808 | 0.9974 | 10.95 (7.08–22.66) |
Giardia intestinalis assemblage B | 81.51 ± 0.08 (81.5–82.0) | 95.77 | − 3.4277 | 0.9969 | 20.92 (15.60–37.15) |
Giardia intestinalis assemblage A | 83.50 ± 0.00 (83.5–83.5) | 96.76 | − 3.4022 | 0.9942 | 20.00 (15.24–33.62) |