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Table 3 List of non-concordant from the 143 samples with their results in both routine diagnostics and the developed 5-Plex-PCR

From: A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites

Samples

Lab routine diagnostica

5-Plex-PCR

Percentage (%)

20

Blastocystis

Blastocystis + Dientamoeba

4.19% due to Dientamoeba

30

Blastocystis + Giardia

Blastocystis+ Giardia+ Dientamoeba

33

Blastocystis

Blastocystis + Dientamoeba

61

Blastocystis

Blastocystis + Dientamoeba

72

Blastocystis

Blastocystis + Dientamoeba

120

Giardia

Giardia + Dientamoeba

35

Blastocystis + Entamoeba

Blastocystis

4.19% non-concordant

6

Negative

Cryptosporidium

48

Negative

Cryptosporidium

49

Negative

Cryptosporidium

80

Negative

Cryptosporidium

119

Giardia

Negative

  1. aDientamoeba was not included in the laboratory routine diagnostics tests