Table 3 The primers used in this study
Target region | Sequence 5′ to 3′ | Amplicon size (bp) |
|---|---|---|
B fragilis group | F: ATAGCCTTTCGAAAGRAAGAT R: CCAGTATCAACTGCAATTTTA | 495 |
B fragilis | F: TCRGGAAGAAAGCTTGCT R: CATCCTTTACCGGAATCCT | 163 |
All bft gene | F: GGATACATCAGCTGGGTTGTAG R: GCGAACTCGGTTTATGCAGT | 296 |
bft-1 | F: TCTTTTGAATTATCCGTATGCTC R: CTTGGGATAATAAAATCTTAGGGATG | 169 |
bft-2 | F: ATTTTTAGCGATTCTATACATGTTCTC R: GGGCATATATTGGGTGCTAGG | 114 |
bft-3 | F: TGGATCATCCGCATGGTTA R: TTTGGGCATATCTTGGCTCA | 148 |
Flanking region | F: TTCAACCTGATCGATCCGGAAGATCCG R: GCTGGTAGACTACCTGAGTAAGGAGTC | 1600 |
BFT qRT-PCR | F: AAGGGCTGGATGGCTTTACT R: GGGATACATCAGCTGGGTTG | – |
16S qRT-PCR | F: CAGTCTTGAGTACAGTAGAGGTGG R: GTGGACTACCAGGGTATCTAATCC | – |