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Fig. 3 | Gut Pathogens

Fig. 3

From: CagA orchestrates eEF1A1 and PKCδ to induce interleukin-6 expression in Helicobacter pylori-infected gastric epithelial cells

Fig. 3

CagA-eEF1A1 complex induces IL-6 through STAT3 (S727) phosphorylation. a Western blot analysis to assess STAT3 in AGS-shSTAT3 cells (STAT3 knockdown by transduced with Lv-STAT3-shRNA viruses) and AGS-NC cells (negative control, transduced with Lv-con-shRNA viruses). b The levels of IL-6 in AGS-shSTAT3 and AGS-NC infected with blank or NCTC11637 or NCTC11637ΔcagA. c The levels of p-STAT3S727 in AGS-shSTAT3 and AGS-NC cells infected with NCTC11637ΔcagA or NCTC11637 using WB analysis. d The levels of p-STAT3S727 in AGS-C and AGS-sheEF1A1 infected with NCTC11637ΔcagA or NCTC11637 using WB analysis. Error bar represents the SDs of triplicate experiments. Statistical analysis was performed using Student’s t-tests. *P < 0.05; **P < 0.01; ***P < 0.001

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