Fig. 4
CagA-eEF1A1-PKCδ complex increases the amount of p-STAT3S727 in the nucleus. a Co-immunoprecipitation analysis of the precipitates where more PKCδ was recruited by eEF1A1 with the presence of CagA. b Rottlerin (6 µM for 30 min) treated AGS-C and AGS-sheEF1A1 cells were infected with NCTC11637 or NCTC11637ΔcagA bacterial inoculums (MOI = 100) for detection of p-STAT3S727 and CagA by western blot analysis, and the level of IL-6 in the culture supernatant. c The level of IL-6 of the supernatant of rottlerin (6 µM for 30 min) treated AGS-C and AGS-sheEF1A1 cells transduced with Ad-cagA or Ad-GFP (MOI = 50). Error bar represents the SDs of triplicate experiments. *P < 0.05; **P < 0.01; ***P < 0.001