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Fig. 2 | Gut Pathogens

Fig. 2

From: Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin

Fig. 2

Optimization of key parameters of multiplex ARMS PCR assay. a Optimal primer annealing conditions. Mutations 2143G (clarithromycin resistance) and 261A (levofloxacin resistance) were used in the test as a starting input of 108 plasmids for ARMS-PCR. An annealing temperature of 58 °C provided optimal amplification of the mutation sequences. b Specificity of ARMS-PCR for resistance mutations. Wildtype 23S rRNA and gyrA sequences were tested at a starting input of 108 plasmids. Positive control mutations 2143G and 261A mutations were tested at a starting input of 500 plasmids. The ARMS-PCR test was highly specific, only detecting the mutation sequences

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Gut Pathogens

ISSN: 1757-4749