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Fig. 3 | Gut Pathogens

Fig. 3

From: Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin

Fig. 3

Analytical sensitivity of the multiplex ARMS-PCR assay.a Detection of clarithromycin resistance mutations. b Analytical sensitivity. For all the clarithromycin and levofloxacin mutations tested, the minimum detection sensitivity was 50 copies. c Detection of clarithromycin and levofloxacin heteroresistance. For modelling heteroresistance, ARMS-PCR reactions were performed with clarithromycin resistance mutation 2143G and levofloxacin resistance mutation 261A, respectively. LR-1, LR-2 and LR-3 denotes 261A with a starting input of 106, 105 and 104 plasmids, respectively. CR-1, CR-2 and CR-3 denotes 2143G with a starting input of 106, 105 and 104 plasmids, respectively. LR-mix1, LR-mix2 and LR-mix3 indicate 261A:2143G ratios of 1:0.1, 1:0.01 and 1:0.001, respectively. CR-mix1, CR-mix2 and CR-mix3 indicate 2143G:216A ratios of 1:0.1, 1:0.01 and 1:0.001, respectively. At all mutation ratios, both mutation sequences were efficiently amplified, indicating high sensitivity of the test for detecting heteroresistance

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Gut Pathogens

ISSN: 1757-4749