Table 3 :Performance of multiplex ARMS-PCR for detection of antibiotic resistance in the 74 positive samples
Parameters | Clarithromycin resistance | Levofloxacin resistance | Clarithromycin and Levofloxacin resistance | ||||||
|---|---|---|---|---|---|---|---|---|---|
Sequencing | Sequencing | Sequencing | |||||||
+ | – | + | – | + | – | ||||
ARMS-PCR | |||||||||
+ | 53 | 0 | 53 | 50 | 7 | 57 | 30 | 5 | 35 |
_ | 0 | 21 | 21 | 1 | 16 | 17 | 0 | 39 | 39 |
53 | 21 | 74 | 51 | 23 | 74 | 30 | 45 | 74 | |
Sensitivity | 100% | 98.04% | 100% | ||||||
Specificity | 100% | 69.57% | 88.64% | ||||||
PPV | 100% | 89.19% | 93.24% | ||||||
Kappa | 1.00 | 0.73 | 0.86 | ||||||