Fig. 1

Relatedness of mcr-1 variants and characteristics of novel identified mcr-1.26 and mcr-1.27. The image visualizes the relatedness of different mcr-1 allele variants, including the two novel ones described in this study (mcr-1.26 and mcr-1.27), as single-gene phylogenetic tree (a). Further, an excerpt of the first 40 bp of mcr-1 genes were displayed and with bold and red letters the novel properties of mcr-1.26 and mcr-1.27 gene sequences are highlighted (b). In c, the first 20 amino acids of translated mcr-1 genes were shown and with bold and red letters the non-synonymous changes of mcr-1.26 and mcr-1.27 are highlighted. Visualization was realized using iTOL (v 5) [32]