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Table 1 Typing results and gene detections of the E. coli strains 803-18 and 844-18

From: Genome sequences of two clinical Escherichia coli isolates harboring the novel colistin-resistance gene variants mcr-1.26 and mcr-1.27

Strain no. Genotyping/phylogeny Resistance gene detection Antibiotic susceptibility testing Plasmid content analyses Virulence gene detection
E. coli phylogenetic groupa Multilocus sequence type (ST)b complex type (CT)b Resistance genes PCR Resistance genes ResFinderc Resistance to antibioticsd Replicon prediction with PlasmidFinderc Plasmid sizes S1-PFGEe Virulence gene {protein function} VirulenceFinderc
803-18 B1 ST155 CT7500 blaTEM, mcr-1-like blaTEM-1B, mcr-1-like, aadA17-like, strA-like,strB-like, tetA, sul2, dfrA14-like, lnuF AMP, PIP, CST, STRf, NALf, MOX, CIP, TETf, SXT IncFIC(FII), IncFIB(AP001918), IncI1,
IncX4, ColRNAI, Col(MG828)
33 kb, 90 kb, 100 kb gad{glutamate decarboxylase}, iroN{enterobactin siderophore receptor protein}, iss{increased serum survival}, lpfA{long polar fimbriae}
844-18 D ST69 CT7508 blaTEM, mcr-1-like blaTEM-1B, mcr-1-like, aadA1, mph(B), dfrA1, tetA, sul1, catA1-like AMP, PIP, CST, NALf, MOX, CIP, TETf, CMP, SXT IncFII, IncFIB(AP001918), IncX4, Col(BS512), ColRNAI, Col156, Col(MG828) 33 kb, 80 kb, 160 kb air{enteroaggregative immunoglobulin repeat protein}, cma{colicin M}, eilA{Salmonella HilA homolog}, gad{glutamate decarboxylase}, iroN{enterobactin siderophore receptor protein}, iss{increased serum survival}, lpfA{long polar fimbriae}
  1. aPCR according to Clermont et al. [8]; binformation extracted from whole-genome sequence (WGS) data (Illumina, HiSeq), using the SeqSphere+ software suite (v 6.0.0) with integrated MLST and cgMLST schemes (https://enterobase.warwick.ac.uk/species/index/ecoli; http://www.ridom.de/seqsphere/u/Task_Template_Sphere.html); cinformation extracted from WGS data, ResFinder (v 3.2)/PlasmidFinder (v 2.1)/VirulenceFinder (v 2.0) (http://www.genomicepidemiology.org/); dBroth microdilution and automated testing (VITEK 2, card AST N248) according to EUCAST (v 10.0); abbreviations of antibiotics: ampicillin (AMP), piperacillin (PIP), colistin (COL), sulfamethoxaole-trimethoprim (SXT), nalidixic acid (NAL), moxifloxacin (MOX), ciprofloxacin (CIP), oxytetracycline (OTE), streptomycin (STR); eS1-nuclease restriction of whole genomic DNA and pulsed-field gel electrophoresis (PFGE) according to Barton et al. [9]; ffor these substance only epidemiological cut-off values (ECOFFs) are available to separates microorganisms without (wild type) and with acquired resistance mechanisms (non-wild type) to the agent in question (http://www.eucast.org); In bold print: plasmid replicon type (incompatibility group) of plasmids that carry the colistin resistance gene mcr-1-like