Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Table 7 Oligonucleotide primers and conditions used to amplify different virulence marker genes in E. faecalis strains by PCR

Gene	Primer sequence (5′-3′)	Annealing temperature	Amplicon size (bp)	References
ddl_E.faecalis	ATCAAGTACAGTTAGTCTTTATTAG ACGATTCAAAGCTAACTGAATCAGT	49	941	[19]
Esp	AGATTTCATCTTTGATTCTTGG AATTGATTCTTAGCATCTGG	48	510	[19]
asa1	TAGGAGTTGTAGGATTAGCTAC TGTTGTATTCMGCSACTTC	47	677	This study
Ace	GGAATGACCGAGAACGATGGC GCTTGATGTTGGCCTGCTTCCG	58	616	[12]
cyl	ACTCGGGGATTGATAGGC GCTGCTAAAGCTGCGCTT	52	688	[2]
gelE	TATGACAATGCTTTTTGGGAT AGATGCACCCGAAATAATATA	58	213	[2]
efbA	GCACAAGTCCCAAAAGGAGC AAGTGCGGCTTCAGTAAGGG	58	510	This study

esp, Enterococcal surface protein; asa1, Aggregation substance; ace, Adhesion of collagen of enterococci; cyl, Cytolysin; gelE, Gelatinase; efbA, Pav A-like fibronectin-binding protein

ISSN: 1757-4749