Fig. 5
From: Eubacterium rectale contributes to colorectal cancer initiation via promoting colitis
NF-κΒ p65 expression in HCoEpiC and NCM460 cells. a Immunocytochemistry results. There is obviously expression in nuclei of HCoEpiC and NCM460 cells treated by 20 ng/ml of LPS extracted from E. rectale for 2.5 h but not F. nucleatum. Untreated cells were used as control group and exhibited p65 expression in plasma. b Western blot results. 1 and 4: No treated cells; 2 and 5: 20 ng/ml of F. nucleatum LPS treatment; 3 and 6: 20 ng/ml of E. rectale LPS treatment. Two cell lines were measured including: 1–3: HCoEpiC; 2–6: NCM460. c The densitometric analysis bar diagram of the results. p65 expression level was normalized by Histone H3 expression level. IKKα and IκBα expression level were normalized by β-actin expression level. Columns represent the mean from three independent experiments and bars represent standard deviations. *P < 0.05, significantly different from control group