Table 1 Sequences and house-keeping genes
Primers | Source | Forward sequence | Reverse sequence |
|---|---|---|---|
Col1a1 | Mouse | GAGTACTGGATCGACCCTAACCAA | ACACAGGTCTGACCTGTCTCCAT |
Col3a1 | Mouse | GCCCACAGCCTTCTACAC | CCAGGGTCACCATTTCTC |
Fn1 | Mouse | CGAAGCCGGGAAGAGCAAG | CGTTCCCACTGCTGATTTATCTG |
Vim | Mouse | CCGTTCAAGGTCAAGACGTGCC | AGGAGGCCGAAAGCACCCTGC |
Il1b | Mouse | AGCTCTCCACCTCAATGGAC | AGGCCACAGGTATTTTGTCG |
Il6 | Mouse | TACACATGTTCTCTGGGAAATCGT | AAGTGCATCATCGTTGTTCATACA |
Il12b | Mouse | GGAAGCACGGCAGCAGAAT | GGCGGGTCTGGTTTGATG |
Il17a | Mouse | GCAAGAGATCCTGGTCCTGA | AGCATCTTCTCGACCCTGAA |
Ifng | Mouse | CAGCAACAGCAAGGCGAAA | CTGGACCTGTGGGTTGTTGAC |
Ccl4 | Mouse | CCACTTCCTGCTGTTTCTCT | TTGGTCAGGAATACCACAGC |
Adgre1 | Mouse | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
Ly6g | Mouse | TGGACTCTCACAGAAGCAAAG | GCAGAGGTCTTCCTTCCAACA |
Cd3e | Mouse | ATGCGGTGGAACACTTTCTGG | GCACGTCAACTCTACACTGGT |
Tbx21 | Mouse | TTCCCATTCCTGTCCTTCAC | CCACATCCACAAACATCCTG |
Gata3 | Mouse | GGAAACTCCGTCAGGGCTA | AGAGATCCGTGCAGCAGAG |
Rorc | Mouse | GAAAGCAGGAGCAATGGAAG | GATGGAAAGCCAGTTCCAAA |
Polr2a | Mouse | GGTGCTGTGGGTACGGATACA | CCCACAACCAGCTATCCTCAA |
FN1 | Human | GATGCTCCCACTAACCTCCA | CGGTCAGTCGGTATCCTGTT |
VIM | Human | GCAGGCTCAGATTCAGGAACA | GTGAGGTCAGGCTTGGAAACA |
OCLN | Human | AGGACGTGCCTTCACCCCCA | ACCACCGCTGCTGTAACGAGG |
IL1B | Human | GATGCACCTGTACGATCACT | GACATGGAGAACACCACTTG |
IL6 | Human | AGTGAGGAACAAGCCAGAGC | GTCAGGGGTGGTTATTGCAT |
IL12B | Human | CCTGACCATCCAAGTCAAAGAGT | AGGAGCGAATGGCTTAGAACCT |
IL8 | Human | AAATCAGGAAGGCTGCCAAGA | AAGGAACCATCTCACTGTGTGTAAAC |
GAPDH | Human | GACACCCACTCCTCCACCTTT | TTGCTGTAGCCAAATTCGTTGT |