Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt

Table 1 Primers used in this study and their annealing temperature

Category	Target gene	Primers sequences (5′–3′)	PCR type	Amplified segment (bp)	Annealing temperature (˚C)
Stx	stx1	F: ACACTGGATGATCTCAGTGG	Duplex	614	60
	stx1	R: CTGAATCCCCCTCCATTATG		614
	stx2	F: CCATGACAACGGACAGCAGTT		779
	stx2	R: CCTGTCAACTGAGCAGCACTTTG		779
MBLs	bla_IMP	F: CATGGTTTGGTGGTTCTTGT	Uniplex	488	55
	bla_IMP	R: ATAATTTGGCGGACTTTGGC		488	55
	bla_VIM	F: AGTGGTGAGTATCCGACAG		261	52
	bla_VIM	R: ATGAAAGTGCGTGGAGAC		261	52
	bla_NDM-1	F: GGCGGAATGGCTCATCACGA		287	58
	bla_NDM-1	R: CGCAACACAGCCTGACTTTC		287	58
ESBLs	bla_OXA-1 group	F: GGCACCAGATTCAACTTTCAAG	Multiplex	564	61
	bla_OXA-1 group	R: GACCCCAAGTTTCCTGTAAGTG		564
	bla_TEM group	F: CATTTCCGTGTCGCCCTTATTC		800
	bla_TEM group	R: CGTTCATCCATAGTTGCCTGAC		800
	bla_CTX-M-1 group	F: TTAGGAAGTGTGCCGCTGTA		655
	bla_CTX-M-1 group	R: CGGTTTTATCCCCCACAAC		655

Stx: Shiga like toxin producing genes; MBLs: metallo-β-lactamase (carbapenemase)-producing genes; ESBLs: extended-spectrum β-lactamase producing genes

ISSN: 1757-4749