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Table 4 Johne data

From: Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

Animal #

ELISA+/−

IFNγ+/−

Fecal PCR+/−

Phage/qPCR +/−

1j

−

+

+

+

2j

−

+

−

+

3j

−

+

+

−

4j

−

−

−

+

5j

−

−

+

−

6j

−

−

−

+

7j

−

+

−

+

8j

−

+

−

+

9j

−

+

+

+

10j

+

+

+

+

11j

−

+

+

−

12j

−

+

+

−

13j

−

+

−

+

14j

−

+

+

+

15j

−

−

+

+

16j

−

+

+

−

17j

−

+

+

+

18j

−

+

+

+

19j

+

−

+

−

20j

−

+

+

−

21j

+

+

+

+

22j

+

−

+

−

23j

−

+

+

+

24j

−

+

+

+

25j

−

+

−

+

26j

+

−

+

+

27j

−

−

+

+

28j

+

−

+

−

29j

+

−

−

+

30j

+

+

+

+

31j

+

+

+

+

32j

+

+

+

+

% positive (# positive/total tested)

31% (10/32)

69% (22/32)

75% (24/32)

72% (23/32)

  1. ELISA is the least sensitive at demonstrating MAP infection. IFN-γ, Fecal PCR and phage/qPCR have comparable sensitivity. Of the ten animals that were positive on ELISA, 5 were positive by all four methods. Two of the remaining ELISA positives were positive on Fecal PCR