Skip to main content

Table 4 Johne data

From: Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

Animal # ELISA+/− IFNγ+/− Fecal PCR+/− Phage/qPCR +/−
1j + + +
2j + +
3j + +
4j +
5j +
6j +
7j + +
8j + +
9j + + +
10j + + + +
11j + +
12j + +
13j + +
14j + + +
15j + +
16j + +
17j + + +
18j + + +
19j + +
20j + +
21j + + + +
22j + +
23j + + +
24j + + +
25j + +
26j + + +
27j + +
28j + +
29j + +
30j + + + +
31j + + + +
32j + + + +
% positive (# positive/total tested) 31% (10/32) 69% (22/32) 75% (24/32) 72% (23/32)
  1. ELISA is the least sensitive at demonstrating MAP infection. IFN-γ, Fecal PCR and phage/qPCR have comparable sensitivity. Of the ten animals that were positive on ELISA, 5 were positive by all four methods. Two of the remaining ELISA positives were positive on Fecal PCR