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Table 5 Age, plaque numbers and qPCR (+/− IS900) for each animal

From: Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

  Control   Johne
# Age (years) Plaque # qPCR Pos/Neg Total assay # (# Pos) # Age (years) Plaque # qPCR Pos/Neg Total assay# (# Pos)
1c 8 11cal + 4 (1) 1j 8 27 + 7 (6)
2c 8 1000est + 9 (4) 2j 12 312 + 5 (1)
3c 12 3 4 (0) 3j 12 139 8 (0)
4c 12 238 + 4 (1) 4j 9 1000est + 4 (2)
5c 5 688 + 8 (7) 5j 9 25 8 (0)
6c 5 29 + 11 (3) 6j 9 30 + 12 (2)
7c 4 10 4 (0) 7j 5 189 + 4 (2)
8c 4 492 + 16 (3) 8j 5 98 + 12 (3)
9c 3 1 4 (0) 9j 13 14 + 4 (1)
10c 3 151 5 (0) 10j 4 5 + 4 (1)
11c 3 47 4 (0) 11j 4 8 8 (0)
12c 1.3 4 4 (0) 12j 4 545 5 (0)
13c 2 1cal No DNA No DNA 13j 4 129 + 4 (2)
14c 2 8cal 4 (0) 14j 3 78 + 12 (1)
15c 2 1cal No DNA No DNA 15j 3 38cal + 1 (1)
16c 2 4 4 (0) 16j 3 59 12 (0)
17c 1.3 6 4 (0) 17j 3 26 + 4 (1)
      18j 3 214 + 5 (1)
      19j >11 48 12 (0)
      20j 7 449 3 (0)
      21j 5 91 + 14 (7)
      22j 5 135 2 (0)
      23j 5 308 + 15 (1)
      24j 5 321 + 14 (3)
      25j 4 97 + 11 (3)
      26j 11 145 + 4 (4)
      27j 10 1000est + 8 (5)
      28j 5 359 2 (0)
      29j 9 229 + 12 (3)
      30j 7 17 + 4 (2)
      31j 5 64 + 12 (3)
      32j 3 5 + 4 (4)
   Mean = 159±71 += 35% (6/17)     Mean = 194±44 += 72% (23/32)  
  1. The count number of plaques (mean ± SEM) presented for 10% of the PBMC’s harvested from 8 ml whole blood following Ficoll gradient. When no plaques were seen, the number presented as “cal” (see Materials and methods). The number of individual qPCR replicates are presented as the total for a given animal with the number of positives in adjacent parentheses (see Materials and methods). “No DNA” indicates that there were so few plaques, that no DNA was extracted. In the Control animals, MAP + animals had significantly more plaques than MAP- animals (Fig 3)