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Table 5 Age, plaque numbers and qPCR (+/− IS900) for each animal

From: Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

 

Control

 

Johne

#

Age (years)

Plaque #

qPCR Pos/Neg

Total assay # (# Pos)

#

Age (years)

Plaque #

qPCR Pos/Neg

Total assay# (# Pos)

1c

8

11cal

+

4 (1)

1j

8

27

+

7 (6)

2c

8

1000est

+

9 (4)

2j

12

312

+

5 (1)

3c

12

3

4 (0)

3j

12

139

8 (0)

4c

12

238

+

4 (1)

4j

9

1000est

+

4 (2)

5c

5

688

+

8 (7)

5j

9

25

8 (0)

6c

5

29

+

11 (3)

6j

9

30

+

12 (2)

7c

4

10

4 (0)

7j

5

189

+

4 (2)

8c

4

492

+

16 (3)

8j

5

98

+

12 (3)

9c

3

1

4 (0)

9j

13

14

+

4 (1)

10c

3

151

5 (0)

10j

4

5

+

4 (1)

11c

3

47

4 (0)

11j

4

8

8 (0)

12c

1.3

4

4 (0)

12j

4

545

5 (0)

13c

2

1cal

No DNA

No DNA

13j

4

129

+

4 (2)

14c

2

8cal

4 (0)

14j

3

78

+

12 (1)

15c

2

1cal

No DNA

No DNA

15j

3

38cal

+

1 (1)

16c

2

4

4 (0)

16j

3

59

12 (0)

17c

1.3

6

4 (0)

17j

3

26

+

4 (1)

     

18j

3

214

+

5 (1)

     

19j

>11

48

12 (0)

     

20j

7

449

3 (0)

     

21j

5

91

+

14 (7)

     

22j

5

135

2 (0)

     

23j

5

308

+

15 (1)

     

24j

5

321

+

14 (3)

     

25j

4

97

+

11 (3)

     

26j

11

145

+

4 (4)

     

27j

10

1000est

+

8 (5)

     

28j

5

359

2 (0)

     

29j

9

229

+

12 (3)

     

30j

7

17

+

4 (2)

     

31j

5

64

+

12 (3)

     

32j

3

5

+

4 (4)

  

Mean = 159±71

+= 35% (6/17)

   

Mean = 194±44

+= 72% (23/32)

 
  1. The count number of plaques (mean ± SEM) presented for 10% of the PBMC’s harvested from 8 ml whole blood following Ficoll gradient. When no plaques were seen, the number presented as “cal” (see Materials and methods). The number of individual qPCR replicates are presented as the total for a given animal with the number of positives in adjacent parentheses (see Materials and methods). “No DNA” indicates that there were so few plaques, that no DNA was extracted. In the Control animals, MAP + animals had significantly more plaques than MAP- animals (Fig 3)