Fig. 3

Specific interaction of miR-615-3p with ST3GAL2 confirmed by RNAi and dual luciferase reporter assay. A, B RT-qPCR analysis for ST3GAL2 transcription in CMT 93 cells transfected with miR-615-3p mimics and nonsense miRNA as control. Fold change of ST3GAL2 and miR-615-3p was calculated relatively to nonsense control and normalised with HPRT, SDHA, B2M and miR-16, miR-145, respectively. Datasets are presented as means of three biological samples and double measurements ± SD. C Identified target sites of ST3GAL2 and miR-615-3p were analysed with RNAhybrid. D Relative luciferase activity was determined to the nonsense miRNA mimic serving as control. Luciferase signal was assayed at 24 h post-transfection. Dataset was plotted as average values of at least four biological replicates with standard deviation from three measurements. Columns show the mean ± SD (bars). Asterisks represent a statistical significance compared to negative controls at each time point. *P ≤ 0.05, ****P ≤ 0.0001, unpaired t-test