Virulence among different types of hypervirulent Klebsiella pneumoniae with multi-locus sequence type (MLST)-11, Serotype K1 or K2 strains

Table 3 Comparison of the phenotypic and virulence differences of one select isolate from each group of HvKp and their virulence gene knockout mutants

Serotypea	MLST	Strain	Serum assay: R, I, S (grade)	String test (≥ 5 mm)	LD₅₀ (CFU)
K1	ST23	wt	R (6)	+	3.98 × 10²
		ΔiucA	R (6)	+	1.0 × 10⁴
		Δcps	R (6)	− ve	≥ 10⁷
		ΔiucAΔcps	R (6)	− ve	≥ 10⁷
K2	ST65	wt	R (6)	+	2.4 × 10²
		ΔiucA	R (5)	+	4.17 × 10⁴
		Δcps	R (6)	− ve	≥ 10⁷
		ΔiucAΔcps	R (6)	− ve	≥ 10⁷
K20^a	ST11	wt	S (1)	− ve	≥ 10⁷
		ΔiucA	S (1)	− -ve	≥ 10⁷
		Δcps	S (1)	− ve	≥ 10⁷
		ΔiucAΔcps	S (1)	− ve	≥ 10⁷
K47^a	ST11	wt	S (1)	− ve	≥ 10⁷
K64^a	ST11	wt	S (1)	− ve	≥ 10⁷

^aOne isolate of each serotype K1, K2 and MLST11, was selected as a representative isolate for each group to generate virulent gene knockout mutants

ISSN: 1757-4749